MS12B – Estimation of uncertainties in microbiology of chemical disinfectants

(no prerequisites necessary – training only in english)

• Know the vocabulary and concepts of uncertainty in microbiology in the field of chemical antiseptics and disinfectants

• Know how to estimate the uncertainty associated with an analytical result in microbiology in the field of chemical antiseptics and disinfectants (quantitative determinations).

  • Concept of uncertainty and notions of metrology:
    • The metrological characteristics of an analytical method in microbiology.
    • What is uncertainty? Why calculate them?
    • Taking into account uncertainties in the interpretation of the result.
  • Simple statistical tools: dispersion and position parameters, distribution laws
  • Estimating uncertainties in microbiology:
    • Overview of existing standards
    • Methodologies for estimating measurement uncertainties for quantitative determinations in the field of chemical antiseptics and disinfectants: methods based on EN ISO 19036, and on ISO 5725-2 and ISO 21748.
  • PRACTICAL:
    • Application exercises of both uncertainty calculation methodologies based on ISO 5725-2 and NF EN ISO 19036.
    • Construction by trainees of calculation files for the estimation of uncertainties in Excel.

At the end of this training, the participant will be able to estimate the uncertainties on the results of microbiology analyses according to main standards (ISO 5725-2 and NF EN ISO 19036).